Working Group meetings open to all attendees!
DaysDays
HrsHours
MinsMinutes
SecsSeconds
Participants can view recorded presentations and listen to discussions after the meeting. See the program for instructions.
AGENDA for the Veterinary Drug Residue Working Group Meeting
10:45 – 11:05 am
Progress of the VDR Collaborative Study
Maïwenn Le Floch, Anses-Fougères, National and EU Reference Laboratory for VDR in Food; Fougères, France; maiwenn.lefloch@anses.fr
Thanks to the evolution of instruments that increase in sensitivity and detection capacity, it is becoming interesting to implement multi-class methods of several hundred substances in control laboratories. Developments of these kind of methods are already a topic of concern worldwide, and it is relevant to highlight their use oriented towards official control. It is with this in mind that the NACRW Vet Drug Residue Working Group started to talk about the organization in 2019 of an inter-laboratory collaborative study to evaluate the screening practices for veterinary drug residues carried out using various new generation mass spectrometry devices. The main goal of this study is to evaluate and establish general identification criteria for LC-MS methods using empirical data to minimize rates of false positives and negatives. Three “Rounds” are planned by three different leading labs, each of them selecting two different commodities. In each Round, the study coordinator has to prepare and ship a tray of autosampler vials consisting of spiked final extracts and calibration standards. The receiving laboratories have to analyze the extracts in a prescribed sequence with their own method(s) that should include the 30 targeted drug analytes using LC-MS/MS and/or HRMS instrumentation. The organization of the Round 1 started in the fall of 2021 led by the laboratory of Anses-Fougères in France. During this meeting will be presented the evolution of the study over the past twelve months.
11:05 – 11:25 am
Stability of Veterinary Drugs
Steven J. Lehotay, U.S. Department of Agriculture, Agricultural Research Service; Wyndmoor, PA 19038; USA. email: steven.lehotay@usda.gov A stability study was conducted for 27 of the 30 veterinary drugs targeted in the collaborative study being conducted by the Veterinary Drugs Working Group. The drug analytes were monitored over the course of a month at 10, 100, and 1,000 ng/g sample equivalents in the diluted aqueous final extracts for Round 2 in the study (chicken egg and 1/1 bovine liver/kidney). Storage temperatures were -80, -18, 8, and 20 degrees C in the dark. Nearly all of the drugs were stable for at least 30 days in frozen conditions, and few of the analytes were stable at room temperature, as will be shown in the brief presentation.
11:25 - 11:45 am
Insights into the new EU Implementing Regulation for Analytical Methods Performance for Veterinary Drug Residue Official Control in Food: CIR (EU) 2021/808
Eric Verdon, Anses-Fougères, National and EU Reference Laboratory for VDR in Food; Fougères, France; eric.verdon@anses.fr
In June 2021 was enforced into the EU a new Commission Implementing Regulation dedicated to update and repeal the old Decisions (EC) 2002/657 and (EC) 98/179 as regard to the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results as well as on the methods to be used for sampling. This presentation will aim at giving some insights on the new approaches inserted in this document and at comparing criteria with those from the 20-year-old former regulation.
11:45 am – 12:15 pm
Open Forum: Question and Answer Session for NACRW Veterinary Drugs Working Group
Sherri Turnipseed, FDA, Animal Drugs Research Center, Denver, CO, USA A part of the 2022 Annual Meeting of the NACRW Veterinary Drugs Working Group there will be an open forum intended to facilitate discussion among the audience and core members of the Veterinary Working Group. Questions relating to the ongoing collaborative study and other issues of interest to the group will be included. All interested attendees are welcome to participate in the on-line discussion.
Regulatory food laboratory participation is requested in an international collaborative project. The goal is to evaluate identification criteria using LC-MS (triple quadrupole and/or high resolution) for multi-veterinary drug residues analysis.
For more information see the Letter of Invitation below.
Monday, July 26, 2021
1:15 - 3:15 pm
Presentations (P1, P2, P3, P4) and Open Forum discussed the optimal MS-based analyte identification criteria. A collaborative study of multiple matrices and analytes is being planned to evaluate analyte identification criteria to minimize the rate of false positives and false negatives.
2022 MEETINGS
Virtual meeting
Planned program for July 2022 WG Meeting; presented results of stability studies for Round 2; discussed shipping of test materials and alternatives to reduce costs; collaborated on forms and reporting spreadsheets to harmonize for all 3 rounds.
Virtual meeting
Discussed July 2022 Virtual meeting; updated information on potential collaborators; revised some study parameters; discussed documentation including instructions and spreadsheets; discussed the delivery of test materials.
Virtual meeting
Reviewed responses from possible collaborators, addressed their questions and established criteria for accepting laboratories to participate in the study. Evaluated stability studies.
PRIOR YEAR'S MEETINGS
Reviewed study parameters, discussed introductory letter and "Call for Participants"; began developing a list of international laboratories who might participate; prepare protocol for first round.
Discussed introductory letter and "Call for Participants"; began developing a list of official control international laboratories who might participate; prepare protocol for first round.
Draft introductory letter reviewed; added international, E.U. and U.S. labs to invitation list; reviewed website content, drafted content for Meeting at a Glance, reviewed collaborative study timeline, began preparing protocol for first round.
June 2, 2021
Finalize list of potential participants and letter advertising the study. Begin drafting protocol for 1st round of collaborative study. Approve abstract and agenda for July Working Group annual meeting.
Finalize plans for NACRW Annual Meeting Working Group presentations and discussion forum. Review letter of invitation to potential study participants.
Review of the November invitations sent to a list of ca 90 potential study participants from official control labs - Collation of responses of all interested labs has been starting - Round 1 for Meat and Milk is launched at the organizer lab at Anses-Fougeres with preparing pilot milk-meat extracts materials for stability study.
Review performance requirements for multi-residue methods.
Discuss possible collaborative study.
Subgroup reviews proposed collaborative study protocol.
Subgroup proposes top 30 analytes to be studied in collaborative study.
Subgroup discusses details of proposed collaborative study including instruments, matrices, number of replicates, laboratories willing to prepare samples and data to be provided by participating laboratories.
NACRW meeting cancelled.
Subgroup meets to continue discussion of collaborative study specifics.
Subgroup discusses the analytes and concentrations for each matrix
Subgroup assigns the collaborative study threshold concentrations for Top30 analytes in matrices (eggs, kidneys, meats, milks, livers, fishes).
Met at NACRW. Discuss future work to evaluate the performance of multi-analyte for regulatory laboratories.
Met at AOAC. New members identified. Discuss definition of multi-residue/multi-class methods. Propose subgroup to develop a collaborative study.
PLEASE CONTACT THE CHAIRS FOR MORE INFORMATION
Eric Verdon,
Sherri Turnipseed
Goals of the NACRW Veterinary Drugs Working Group
Copyright © 2022 (FLAG Works, Inc./NACRW) All Rights Reserved.